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  • Pre-recorded Live DEMO
  • Pre-recorded Live Demo

Pre-recorded Live Demo

This is a pre-recorded live demo with a relatively detailed walk-through of the HPLC operation.



Previous Cannabis HPLC Analyzer with Autosampler Intro
  • 1. Intro & Theory

    • 1.1 How to use this knowledge base for training purposes
    • 1.2 Certificate of Training Completion
    • 1.3 HPLC Basics

      • 1. What is Chromatography
      • 2. HPLC Principle
      • 3. HPLC System Design
      • 4. Solvents
      • 5. Pump
      • 6. Injector
      • 7. Column
      • 8. Detector
      • 9. Theory of Separation
      • 10. What affects separation?
      • 11. Issues with peaks
      • 12. HPLC vs UHPLC
    • 1.4 About Cannabinoids

      • Learn about major cannabinoids
    • 1.5 HPLC Comparison

      • Comparison of HPLCs used for cannabinoids testing
  • 2. Getting started with HPLC

    • 2.1 Connecting your HPLC

      • Storage/Safety/Disposal
      • Setting up your HPLC
      • Included Accessories
      • Things that can go wrong when installing HPLC
      • Computer Requirements
    • 2.2 CTI HPLC Software

      • HPLC Software Installation
      • USB cable driver installation
      • HPLC Software walk-through
    • 2.3 CTI LIMS

      • CTI LIMS – What is New?
      • 1. CTI LIMS Installation
      • 2. CTI LIMS Activation
      • 3. CTI LIMS Walk-through
      • 4. Calibration Curves Import
      • 5. Making Log Updates
      • 6. Batch Report Generation
      • 7. Making report adjustments
      • 8. Upgrading CTI LOG into CTI LIMS
      • 9. CTI LIMS Customization
    • 2.4 Analytical balance

      • 1. Setting up analytical balance
      • 2. Connecting electronic balance to PC
    • 2.5 Microsyringes & pipettes

      • How to use microsyringes & pipettes
      • Cleaning glassware
  • 3. HPLC Operation

    • 3.1 Process Work Flow

      • Process Flow Chart
    • 3.2 Mobile phase

      • HPLC Solvents
      • How to prepare mobile phase
      • Challenges with mobile phase
      • Storage
    • 3.3 HPLC Start-up & Column conditioning

      • Your first HPLC run
      • Daily HPLC start-ups
      • What can go wrong when starting HPLC
    • 3.4 Sample receiving

      • Sample receiving and logging
    • 3.5 Sample extraction

      • Cannabinoids Extraction
      • Types of samples and extraction best practices
      • Extraction challenges
      • Dilution best practices
    • 3.6 Injection & measurement

      • Sample Injection
      • Things to watch for when injecting sample
    • 3.7 Data processing & report generation

      • Data processing & lab reports
      • Ideal chromatogram settings
      • Things to be aware of when creating lab reports
      • Testing beverages
    • 3.8 HPLC shut-down

      • How to properly shut down HPLC
    • 3.9 Validating your HPLC

      • Commercial Standards
      • HPLC validation with commercial standards
  • 4. Troubleshooting

    • 4.1 Typical issues and how to solve them

      • Typical issues and how to solve them
      • Pressure Issues Intro
    • 4.2 Low Pressure Issues

      • Checking the check valves
      • Low pressure due to leaks
      • Solvents incompatibility
    • 4.3 High Pressure Issues

      • Plugged guard column
      • Plugged main separation column
    • 4.4 Bubbles in the system – causes and impact

      • Bubbles causes and how to deal with them
    • 4.5 Experiencing leaks

      • How to recognize leaks and how to fix them
    • 4.6 Retention times shift

      • Retention times shift
      • How to adjust retention times and save new method
    • 4.7 Issues with Lab Report

      • When I print lab report into PDF it doesn`t fit the page
    • 4.8 Broken connection message during analysis

      • USB Power management
      • Broken USB connector
  • 5. Maintenance

    • 5.1 Maintenance of column

      • Column cleaning and conditioning
      • When to replace column?
      • How to store column for a prolonged period of time?
  • 6. Autosampler

    • 6.1 Autosampler Installation

      • Autosampler Hardware Installation
      • Autosampler Software Installation
    • 6.2 Autosampler Operation

      • Autosampler Operation
      • HPLC Autosampler Settings
    • 6.3 Data Processing & Reports

      • Autosampler Data Processing & Report Generation
    • 6.4 Step-by-step Guide

      • Autosamler Testing Guide
  • 7. Terpenes

    • 7.1 Installation

      • 1. Column Installation
      • 2. How to use autopipette
      • 3. Mobile Phase
      • 4. HPLC Setup & 1st Run
      • 5. Sample Preparation
      • 6. Spike & Sample Spiking
    • 7.2 Software

      • Terpenes Software
      • Terpenes Retention Times
      • HPLC method files
    • 7.3 Data Analysis

      • 1. Terpenes Data Analysis
      • 2. Data Export
      • 3. Reports Generation
      • 4. Terps Report Settings
    • 7.4 Maintenance

      • Column Maintenance
  • 8. Kratom

    • 8.1 How to test Kratom

      • Articles coming soon
  • 9. Cloud-based Multi-user mode

    • 9.1. How does it work?

      • Why is this useful?
      • How does it work?
    • 9.2. Software Setup

      • 1. Linking your computers to OneDrive
      • 2. CTI HPLC Software – multi-user mode with files hosted on OneDrive
      • 3. Download CTI Log multi-user version
      • 4. CTI Log Multi-User Installation on OneDrive
      • 5. CTI Log Installation on Other computers
      • 6. Licence Key Instructions
  • 10. Advanced troubleshooting

    • 10.1. Panel Removal

      • 1. Right Side Panel Removal
      • 2. Left Side Panel Removal
    • 10.2. Problem Diagnostics

      • 1. Low or Zero Light Reference Signal
      • 2. Broken Communication
      • 3. The Initial Self-Test Does Not Complete
    • 10.3. Part Replacement

      • 1. UV Lamp Replacement
      • 2. Switching Power Supply Replacement
      • 3. Motherboard Replacement
      • 4. Communications Board Replacement
      • 5. Grating Lead Screw Maintenance
  • Downloads

    • Software

      • CTI HPLC Software used to operate HPLC
      • CTI Log used to generate reports
    • Manuals

      • Operating Manual
    • Guides complementing instruction videos

      • Guides complementing training videos
    • Brochures

      • Brochures
    • Technical Datasheets

      • Technical Datasheets
    • Application Notes

      • Application Notes
    • Safety Datasheets

      • Safety Datasheets
    • Standard Operating Procedure (SOP)

      • Standard Operating Procedure (SOP)
  • Videos

    • Intro Videos

      • Hemp and Cannabis HPLC Analyzer Introduction
      • Operation Demo – short version
      • Cannabis HPLC Analyzer with Autosampler Intro
    • Pre-recorded Live DEMO

      • Pre-recorded Live Demo

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